Physarum polycephalum from sclerotia ("Mazie" finds a new home)

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John
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Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Fri Dec 08, 2017 9:23 am

A simple non-sterile method.

What you need
1) a plastic dish (petri dish is ideal),
2) water (tap or distilled)
3) kitchen roll or filter paper
4) Physarum polycephalum sclerotia (ideally less than 1 year old)
5) Porridge oats (not instant porridge)


Method.
1) Cut a paper disc to fit the bottom of your dish. Add tap water and drain.
2) Place a piece of dried sclerotia in the centre
3) Place a whole porridge oat nearby
4) Add lid and keep in the dark (a drawer or cupboard) at room temperature.
5) Shower and feed daily (see below).

I was very pleased to find this after only 24 hours.
Physarum_polycephalum1000.jpg
Daily maintenance
1) Shower. Add a small amount of water to the dish, swirl around a little and then drain off excess.
2) Place an oatflake near the growing front of the plasmodium.

Weekly maintenance
1) Once a week, change the bedding - put a fresh piece of paper near the slime with fresh food on top, your physarum will move across and you can dispose of the old paper.

Dividing your colony
You can divide your colony at any time by moving colonised oats to a new container or cutting out a piece of slime covered paper.

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John
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Re: Physarum polycephalum from sclerotia

Post by John » Fri Dec 08, 2017 9:39 am

I placed an oat near the growing edge yesterday afternoon and it was engulfed within the hour. The plasmodium moves much more quickly than I was expecting.
Physarum_polycephalum_48hours_crop_1000_withtext.jpg
Day 2:
I am going to need some larger containers very soon and think of some experiments. This plasmodia was named "Mazie" by it's previous owner :lol: (thanks Ian).

I will try to produce sclerotia in the next few months and make some available to people in the UK (and maybe Europe) via this forum. Please PM me if you would like some and I will try to help.

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Sat Dec 09, 2017 10:35 am

Day3: Mazie plans her escape
Day3_1000.jpg

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Sat Dec 09, 2017 10:42 am

Moving to a larger house
1) Two plastic boxes (One fits inside the other).
2) Kitchen roll (I used the stronger variety which maintains it's strength when wet. "Plenty" brand in the UK)
Box1b_1000.jpg
Smaller box placed upside down in larger box. Kitchen roll is folded down both sides and under the smaller box so it can wick moisture from the base of the larger box
Box2b_1000.jpg
Piece of kitchen roll folded in half longways and placed on the top.
Box3_1000.jpg
Water sprayed over tissues and Mazie's petri dish added to top (lid removed).
Box4_1000.jpg
Lid placed on larger box and stored in the dark.
The idea is to put water into the base of the box to provide moisture by capilliary action and reduce the need for daily "showers".

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Sun Dec 10, 2017 1:36 pm

Day 4: Settling in
Box5_Day4_1000.jpg

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Mon Dec 11, 2017 6:58 pm

Day 5. The petri dish is empty and has been removed, leaving Mazie free to roam.
Box5_Day5_1000.jpg

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Wed Dec 13, 2017 7:02 pm

Day6
Day6_1000.jpg
Day 7: I was not expecting this already... :o
Day7a_1000.jpg

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Re: Physarum polycephalum from sclerotia ("Mazie" finds a new home)

Post by John » Thu Dec 14, 2017 4:32 pm

2017-12-14-13.54.12 ZS PMax_1000.jpg
Cross-eyed stereo pairs
2017-12-14-14.22.23 ZS stereo_000_1000.jpg
Physarum_polycephalum1_1000.jpg

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